03 MARCH 2025

Syphilis remains a significant public health concern, and serological testing plays a central role in its diagnosis and follow‑up. Spinreact introduces RPR‑Carbon, a robust non‑treponemal test designed for the qualitative and semi‑quantitative detection of plasma reagins in human serum or plasma.

Principle of the Method

The RPR‑Carbon test is based on a slide agglutination technique.
Carbon particles coated with a lipid complex—including cardiolipin, lecithin, and cholesterol—react with reagins, antibodies produced by the immune system in response to tissue damage caused by Treponema pallidum, the agent responsible for syphilis.

Visible agglutination indicates a reactive result.
Because titration is possible, the method is also useful for monitoring disease evolution and the patient’s response to antibiotic therapy.

Reagents Included

Each kit contains:

• RPR‑Carbon reagent (carbon particles coated with lipid complex in phosphate buffer, pH 7.0)
• Positive control (reagin titer ≥1/4, red cap)
• Negative control (animal serum, blue cap)
• Disposable slides
• Dispensing vial and needle (depending on format)

These components ensure consistency, reliability, and reproducibility across tests.

Available Formats

Spinreact offers two kit sizes suitable for diverse laboratory needs:

• RPR‑Carbon — 150 tests (Ref. 1200401)
• RPR‑Carbon — 500 tests (Ref. 1200402)

Both kits include all necessary reagents, controls, and accessories.

Storage and Stability

All components remain stable until the expiration date when stored tightly closed at 2–8°C and protected from contamination.
The reagent must not be frozen, as this would alter its functionality.
Gentle homogenization before use ensures optimal reagent performance.

Result Interpretation

The assay provides straightforward macroscopic interpretation with three outcome categories:

• Reactive (R): medium or large visible clumps
• Weakly Reactive (WR): small clumps
• Non‑Reactive (N): absence of clumping

In semi‑quantitative testing, the titer corresponds to the highest dilution showing visible agglutination.

Spinreact’s RPR‑Carbon test offers a dependable, easy‑to‑interpret, and stable non‑treponemal solution for syphilis screening and follow‑up.
With two practical formats—150 and 500 tests—it adapts to laboratories of any size, maintaining Spinreact’s commitment to delivering accurate and accessible diagnostic tools.

We continue to focus on delivering the best diagnostic products to meet our customers’ real needs.

A Reliable Non‑Treponemal Test for Syphilis Diagnosis